The man, believed to be in his 40s, was riding along Great Gutter Lane in Willerby and died in hospital after the smash at lunchtime yesterday.
Driving a Lambretta scooter towards Cottingham, the man is believed to have been turning left into a side road before being struck by a car turning right into the same road.
Humberside Police have appealed for any witnesses to come forward after the crash at 12.45pm.
Officers can be contacted on 0845 6060222.